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Antibodies used for the labeling of cortical synaptic boutons

Journal: The Journal of Neuroscience

Article Title: Impact of the NGF Maturation and Degradation Pathway on the Cortical Cholinergic System Phenotype

doi: 10.1523/JNEUROSCI.1144-11.2012

Figure Lengend Snippet: Antibodies used for the labeling of cortical synaptic boutons

Article Snippet: The sections were washed 3× 10 min in PBS, mounted on gelatin subbed slides, dried overnight at 4°C, and coverslipped with Aqua Polymount (Polysciences). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Anatomical marker Primary antibody Serum used for blocking step and antibody dilutions Secondary antibody VAChT (expressed in cholinergic varicosities) Goat anti-VAChT; 1:10,000 (Millipore), polyclonal Normal rabbit serum (Jackson ImmunoResearch) Biotinylated rabbit anti-goat; 1:200 (Vector Labs) TH (expressed in both dopaminergic and noradrenergic varicosities) Rabbit anti-TH; 1:3000 (Millipore), polyclonal Normal goat serum (Jackson ImmunoResearch) Alexa Fluor 488-conjugated goat anti-rabbit; 1:800 (Invitrogen) DBH (expressed in noradrenergic varicosities) Mouse anti-DBH; 1:25 (MediMabs), monoclonal Normal donkey serum (Jackson ImmunoResearch) Rhodamine Red X-conjugated donkey anti-mouse; 1:200 (Jackson ImmunoResearch) VGluT1 (expressed in glutamatergic varicosities) Mouse anti-VGluT1; 1:100 (characterized in Bell et al. (2006) ; MediMabs), monoclonal Normal donkey serum (Jackson ImmunoResearch) Alexa Fluro 488-conjugated donkey anti-mouse; 1:800 (Invitrogen) GAD 65 (expressed in GABAergic varicosities) Mouse anti-GAD-65; 1:1000 (Millipore), monoclonal Normal donkey serum (Jackson ImmunoResearch) Alexa Fluor 488-conjugated donkey anti-mouse; 1:800 (Invitrogen) Neurotrophic TrkA high-affinity mNGF receptor (expressed on cholinergic axon) Polyclonal goat anti-TrkA; 1:100 (R&D Systems) Normal donkey serum (Jackson ImmunoResearch) Rhodamine Red X-conjugated donkey anti-goat; 1:200 (Jackson ImmunoResearch) Open in a separate window Antibodies used for the labeling of cortical synaptic boutons

Techniques: Labeling, Marker, Blocking Assay, Plasmid Preparation